The Syrian Observatory for Human Rights, an anti-Assad monitoring group, reported that 37 people had been killed in the blast, and more than 40 were wounded in the blast which took place in the village of Hurra.
Situated in the province of Hama, in the east of the country, the village of Hurra is controlled by the Syrian government.
It was not immediately clear if the attack was in any way related to the militant group Isis, which is active in Syria and has seized vast tracts of territory across the border in Iraq in recent weeks.
Syria’s three-year-old conflict began as peaceful protests against the government but has turned into civil war, killing at least 100,000 people according to United Nations estimates.
Rebels have been joined by hardline Islamists, some of them linked to al-Qaeda, who have become increasingly powerful among opposition forces.
Video: Foreign Affairs Correspondent Jonathan Miller answers the key questions on Isis, the extremist Sunni militant group which is leading insurgents in Iraq – but which has also been fighting in Syria.