Fiorentina face Everton with Christian Vieri supported by youngsters Daniel Osvaldo and Zdravko Kuzmanovic.
Cesare Prandelli had hinted he would lead with veteran Vieri in the absence of Adrian Mutu, who is out for a month with a sprained knee.
Giampaolo Pazzini and Daniele Cacia find space on the bench, as does Mario Alberto Santana, who is not 100 per cent fit.
The defence is the first choice line-up generally seen in Serie A, as the Viola attempt to maintain their unbeaten record in the UEFA Cup this season.
Everton are also yet to lose in this competition and the only injury doubt was Mikel Arteta, who is left on the bench.
Joseph Yobo, Steven Pienaar, Tim Cahill and Andrew Johnson have all recovered from a stomach bug and only Johnson fails to find a spot in the starting XI.
As a large number of Everton fans have travelled to Florence without a ticket, the council has erected a giant screen at the Palasport next to the Stadio Franchi.
The weather in Florence is extremely cold, wet and windy with danger of sleet.
Fiorentina: Frey; Ujfalusi, Dainelli, Gamberini, Pasqual; Jorgensen, Montolivo, Donadel; Kuzmanovic, Osvaldo, Vieri
Fiorentina bench: Avramov, Kroldrup, Potenza, Gobbi, Santana, Pazzini, Cacia
Everton: Howard; Hibbert, Lescott, Neville, Yobo, Jagielka, Cahill, Pienaar, Osman, Carsley, Yakubu
Everton bench: Wessels, Baines, Arteta, Gosling, Johnson, Anichebe, Gravesen
